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Capture SOUL Assay



  • Provides natural compounds which fight a negative protein called NFkB. NFkB promotes growth of proinflammatory genes and increases the level of cellular inflammation. Inflammation is one of the greatest threats to our health, according to scientists.*
  • Contains powerful antioxidants, which absorb and protect cells from damaging free-radicals and oxidative stress. Free- radicals are known to contribute to the aging process.*
  • Promotes health and longevity with anti-aging properties at the cellular level. Induces and activates SIRT1, a protein that is believed to play a part in extending lifespan and improving health as one ages.*

Rain International Has Tested Soul with Three Cell-based Tests Which Measure Primary Cellular Mechanisms

Anti-inflammatory, Anti-Aging, and Anti-Oxidant.

In specific, these tests are:


Cellular Anti-Inflammatory Assay (NFkB) determines the anti-inflammatory potential of a given material in human cells. NFkB (Nuclear Factor kappa B), a protein complex that is involved in cellular responses to stimuli such as stress and free radicals, is used as inflammation biomarker.

In this particular NFkB assay, Tumor necrosis factor alpha (TNF-a), a pleiotropic inflammatory cytokine, is introduced to the human cells to trigger cellular inflammation. If an anti-inflammatory material presents in the cellular environment, the material inhibits NFkB activation and the degree of inhibition can be monitored via NFkB expression. NFkB expression level of the human cells, treated with and without test materials, under the stressed condition are therefore monitored and compared.

Maximum percentage of NFkB expression inhibition induced by tested materials is reported.

“The concentration used that induced the maximum inhibition of NFkB expression is also noted.”

• Bioavailability of anti-inflammatory compounds
As much as a 33% inhibition of cellular inflammation
No cellular toxicity at concentrations tested


Cellular Anti-aging Assay (SIRT1) measures the anti-aging ability of a material using SIRT1 production in human cells as a biomarker for anti-aging. SIRT1 is a protein that is believed to play important roles in longevity and reduction of age-related diseases. Previous studies have shown that when mammals age, SIRT1 expression decreases, where induction and activation of SIRT1 has been associated with extended lifespan. These studies have triggered the search for SIRT1 activators that may be used as dietary supplements to promote health and longevity. Cellular Anti-aging Assay (SIRT1 assay) determines the ability of a test material to stimulate SIRT1 protein expression in human cells, which translates to the material’s anti-aging potential. SIRT1 expression level of human cells treated with and without test materials are compared, and maximum percentage of the SIRT1 expression change is reported. The concentration used that induced maximum percentage of the SIRT1 expression change is noted.

• Bioavailability of anti-aging compounds
• 62% increase in the stimulation of the anti-aging enzyme, SIRT1
• No cellular toxicity at concentrations tested


In CAA (Cellular Antioxidant Assay), a fluorescent probe is placed inside of representative human cells, whose loss of fluorescence is an indication of the damage extent from oxygen radical. A material to be tested is incubated with the cells to allow its natural absorption into cells. Then, an oxygen radical inducer is introduced into cellular environment, which triggers the release of oxygen radicals. Without antioxidant material present inside of the cells, oxygen radicals permeate through cell membrane and damage the cells and the marker probe. Such process deters when antioxidant material is present inside of the cells. The cellular antioxidant effect of the test material is then measured by assessing the preservation effect of the marker probe in the presence of the test material absorbed inside of cells.

• Bioavailability of antioxidant compounds
• Significant reduction of free radicals compared to average
• No cellular toxicity at concentrations tested

In each assay, SOUL demonstrated significant positive results at safe concentrations. That is, SOUL significantly reduced radical activity, inhibited the formation of NFkB, and stimulated the expression of SIRT1 in human cell lines. These results represent an important body of pre-clinical evidence which supports the results of other test, such as ORAC5. They also reflect Rain’s ongoing commitment to better understanding Soul’s healthful attributes.

ORAC 5.0

There are five predominant reactive species found in the body: peroxyl radicals, hydroxyl radicals, peroxynitrite, super oxide anion, and singlet oxygen. Total ORACfn provides a measure of the total antioxidant power of a food/nutrition product against the five predominant reactive species. The ORAC result is expressed as micromole trolox equivalency (µmole TE) per gram.

• Measures antioxidant performance against 5 primary radicals
• The industry standard for broad-spectrum antioxidant testing


Brunswick Labs, a global leader in antioxidant R&D and testing, has established a test for ‘Total ORAC’for Food and Nutrition (Total ORACfn). Total ORACfn represents a breakthrough in comprehensive antioxidant testing for food and nutrition products. Total ORACfn delivers valuable, quantitative analysis to evaluate broad-spectrum antioxidant potential of oil and water-soluble ingredients.

Brunswick Lab Receives US Patent for ORAC Analysis Posted on July 11, 2014 Brunswick Lab Receives US Patent for ORAC Analysis  

This Patent reflects the highest quality assurance standards provided by Brunswick Labs, and will benefit all scientists working in the field of antioxidant research.

Brunswick Laboratories, the leading commercial laboratory specializing in the science of antioxidants and oxidative stress, has received a Patent Notice of Allowance from the US Patent and Trademark Office (USPTO) for its well-known ORAC assay. A Notice of Allowance is the formal USPTO notification that an applicant is entitled to a patent under the law.

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